Part:BBa_K5007017:Design

pRS426 F-primer

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 16
  Illegal SpeI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 10
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 16
  Illegal SpeI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 16
  Illegal SpeI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Primer designed for cloning part BBa_K5007007 into the pRS426 plasmid through Gibson Assembly, inside the LacZɑ gene cloning region. This primer linearizes the plasmid, generating the homology regions needed for the assembly method.

Source

This primer was designed internally and synthesized for use in PCR reactions.